2F56
Barnase cross-linked with glutaraldehyde soaked in 6M urea
Summary for 2F56
Entry DOI | 10.2210/pdb2f56/pdb |
Related | 1A2P 2F4Y |
Descriptor | Ribonuclease, UREA, ZINC ION, ... (4 entities in total) |
Functional Keywords | denaturation, lysozyme, barnase, cross-linked crystals, glutaraldehyde, urea, thiourea, bromoethanol, hydrolase |
Biological source | Bacillus amyloliquefaciens |
Cellular location | Secreted: P00648 |
Total number of polymer chains | 3 |
Total formula weight | 37324.56 |
Authors | Prange, T.,Salem, M. (deposition date: 2005-11-25, release date: 2006-04-25, Last modification date: 2023-08-23) |
Primary citation | Salem, M.,Mauguen, Y.,Prange, T. On the edge of the denaturation process: Application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations. Biochim.Biophys.Acta, 1764:903-912, 2006 Cited by PubMed: 16600702DOI: 10.1016/j.bbapap.2006.02.009 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.955 Å) |
Structure validation
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