2F37

Crystal structure of the ankyrin repeat domain of human TRPV2

2F37 の概要

• エントリーDOI: 10.2210/pdb2f37/pdb
• 分子名称: Transient receptor potential cation channel subfamily V member 2, SULFATE ION (3 entities in total)
• 機能のキーワード: ankyrin repeat, membrane protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Multi-pass membrane protein (By similarity): Q9Y5S1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56014.63

構造登録者

McCleverty, C.J. (登録日: 2005-11-18, 公開日: 2006-10-31, 最終更新日: 2024-11-13)

主引用文献

McCleverty, C.J.,Koesema, E.,Patapoutian, A.,Lesley, S.A.,Kreusch, A.
Crystal structure of the human TRPV2 channel ankyrin repeat domain.
Protein Sci., 15:2201-2206, 2006

PubMed Abstract: TRPV channels are important polymodal integrators of noxious stimuli mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common protein-protein recognition domain, is conserved in the N-terminal intracellular domain of all TRPV channels and predicted to contain three to four ankyrin repeats. Here we report the first structure from the TRPV channel subfamily, a 1.7 A resolution crystal structure of the human TRPV2 ARD. Our crystal structure reveals a six ankyrin repeat stack with multiple insertions in each repeat generating several unique features compared with a canonical ARD. The surface typically used for ligand recognition, the ankyrin groove, contains extended loops with an exposed hydrophobic patch and a prominent kink resulting from a large rotational shift of the last two repeats. The TRPV2 ARD provides the first structural insight into a domain that coordinates nociceptive sensory transduction and is likely to be a prototype for other TRPV channel ARDs.

PubMed: 16882997
DOI: 10.1110/ps.062357206

実験手法

X-RAY DIFFRACTION (1.7 Å)