2F2J

Solution structure of [W19K, P20N, V21K]-kalata B1

2F2J の概要

• エントリーDOI: 10.2210/pdb2f2j/pdb
• 関連するPDBエントリー: 2F2I
• 分子名称: Kalata-B1 (1 entity in total)
• 機能のキーワード: cystine knot, circular backbone, beta-hairpin, antimicrobial protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2907.35

構造登録者

Clark, R.J.,Daly, N.L.,Craik, D.J. (登録日: 2005-11-17, 公開日: 2006-01-31, 最終更新日: 2024-10-23)

主引用文献

Clark, R.J.,Daly, N.L.,Craik, D.J.
Structural plasticity of the cyclic cystine knot framework: implications for biological activity and drug design
Biochem.J., 394:85-93, 2006

PubMed Abstract: The cyclotide family of plant proteins is of interest because of their unique topology, which combines a head-to-tail cyclic backbone with an embedded cystine knot, and because their remarkable chemical and biological properties make them ideal candidates as grafting templates for biologically active peptide epitopes. The present study describes the first steps towards exploiting the cyclotide framework by synthesizing and structurally characterizing two grafted analogues of the cyclotide kalata B1. The modified peptides have polar or charged residues substituted for residues that form part of a surface-exposed hydrophobic patch that plays a significant role in the folding and biological activity of kalata B1. Both analogues retain the native cyclotide fold, but lack the undesired haemolytic activity of their parent molecule, kalata B1. This finding confirms the tolerance of the cyclotide framework to residue substitutions and opens up possibilities for the substitution of biologically active peptide epitopes into the framework.

PubMed: 16300479
DOI: 10.1042/BJ20051691

実験手法

SOLUTION NMR