2F1E

Solution structure of ApaG protein

2F1E の概要

• エントリーDOI: 10.2210/pdb2f1e/pdb
• NMR情報: BMRB: 5998
• 分子名称: Protein apaG (1 entity in total)
• 機能のキーワード: apag protein, xanthomonas axonopodis pv.citri, structural genomics, unknown function
• 由来する生物種: Xanthomonas axonopodis pv. citri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14196.69

構造登録者

Contessa, G.,Pertinhez, T.A.,Spisni, A.,Paci, M.,Farah, C.S.,Cicero, D.O. (登録日: 2005-11-14, 公開日: 2006-10-24, 最終更新日: 2024-05-29)

主引用文献

Cicero, D.O.,Contessa, G.M.,Pertinhez, T.A.,Gallo, M.,Katsuyama, A.M.,Paci, M.,Farah, C.S.,Spisni, A.
Solution structure of ApaG from Xanthomonas axonopodis pv. citri reveals a fibronectin-3 fold.
Proteins, 67:490-500, 2007

PubMed Abstract: ApaG proteins are found in a wide variety of bacterial genomes but their function is as yet unknown. Some eukaryotic proteins involved in protein-protein interactions, such as the human polymerase delta-interacting protein (PDIP38) and the F Box A (FBA) proteins, contain ApaG homology domains. We have used NMR to determine the solution structure of ApaG protein from the plant pathogen Xanthomonas axonopodis pv. citri (ApaG(Xac)) with the aim to shed some light on its molecular function. ApaG(Xac) is characterized by seven antiparallel beta strands forming two beta sheets, one containing three strands (ABE) and the other four strands (GFCC'). Relaxation measurements indicate that the protein has a quite rigid structure. In spite of the presence of a putative GXGXXG pyrophosphate binding motif ApaG(Xac) does not bind ATP or GTP, in vitro. On the other hand, ApaG(Xac) adopts a fibronectin type III (Fn3) fold, which is consistent with the hypothesis that it is involved in mediating protein-protein interactions. The fact that the proteins of ApaG family do not display significant sequence similarity with the Fn3 domains found in other eukaryotic or bacterial proteins suggests that Fn3 domain may have arisen earlier in evolution than previously estimated.

PubMed: 17256769
DOI: 10.1002/prot.21277

実験手法

SOLUTION NMR