2F01

Epi-biotin complex with core streptavidin

2F01 の概要

• エントリーDOI: 10.2210/pdb2f01/pdb
• 関連するPDBエントリー: 1MK5
• 分子名称: Streptavidin, BIOTIN, EPI-BIOTIN, ... (5 entities in total)
• 機能のキーワード: protein/ligand interactions, streptavidin, biotin, biotin binding protein
• 由来する生物種: Streptomyces avidinii
• 細胞内の位置: Secreted: P22629
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27816.20

構造登録者

Le Trong, I.,Aubert, D.G.,Thomas, N.R.,Stenkamp, R.E. (登録日: 2005-11-10, 公開日: 2005-11-29, 最終更新日: 2023-08-23)

主引用文献

Le Trong, I.,Aubert, D.G.,Thomas, N.R.,Stenkamp, R.E.
The high-resolution structure of (+)-epi-biotin bound to streptavidin.
Acta Crystallogr.,Sect.D, 62:576-581, 2006

PubMed Abstract: (+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral center at atom C2. This could lead to a difference in the mode of binding of (+)-epi-biotin to streptavidin, a natural protein receptor for (+)-biotin. Diffraction data were collected to a maximum of 0.85 Angstrom resolution for structural analysis of the complex of streptavidin with a sample of (+)-epi-biotin and refinement was carried out at both 1.0 and 0.85 Angstrom resolution. The structure determination shows a superposition of two ligands in the binding site, (+)-biotin and (+)-epi-biotin. The molecules overlap in the model for the complex except for the position of S1 in the tetrahydrothiophene ring. Differences in the conformation of the ring permits binding of each molecule to streptavidin with little observable difference in the protein structures at this high resolution.

PubMed: 16699183
DOI: 10.1107/S0907444906011887

実験手法

X-RAY DIFFRACTION (0.85 Å)