2EZQ

SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 11-20 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES

2EZQ の概要

• エントリーDOI: 10.2210/pdb2ezq/pdb
• 分子名称: GP41 (1 entity in total)
• 機能のキーワード: virus envelope protein, siv gp41 ectodomain, viral protein
• 由来する生物種: Simian immunodeficiency virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 43293.80

構造登録者

Caffrey, M.,Gronenborn, A.M.,Clore, G.M. (登録日: 1998-05-20, 公開日: 1998-10-14, 最終更新日: 2024-05-01)

主引用文献

Caffrey, M.,Cai, M.,Kaufman, J.,Stahl, S.J.,Wingfield, P.T.,Covell, D.G.,Gronenborn, A.M.,Clore, G.M.
Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41.
EMBO J., 17:4572-4584, 1998

PubMed Abstract: The solution structure of the ectodomain of simian immunodeficiency virus (SIV) gp41 (e-gp41), consisting of residues 27-149, has been determined by multidimensional heteronuclear NMR spectroscopy. SIV e-gp41 is a symmetric 44 kDa trimer with each subunit consisting of antiparallel N-terminal (residues 30-80) and C-terminal (residues 107-147) helices connected by a 26 residue loop (residues 81-106). The N-terminal helices of each subunit form a parallel coiled-coil structure in the interior of the complex which is surrounded by the C-terminal helices located on the exterior of the complex. The loop region is ordered and displays numerous intermolecular and non-sequential intramolecular contacts. The helical core of SIV e-gp41 is similar to recent X-ray structures of truncated constructs of the helical core of HIV-1 e-gp41. The present structure establishes unambiguously the connectivity of the N- and C-terminal helices in the trimer, and characterizes the conformation of the intervening loop, which has been implicated by mutagenesis and antibody epitope mapping to play a key role in gp120 association. In conjunction with previous studies, the solution structure of the SIV e-gp41 ectodomain provides insight into the binding site of gp120 and the mechanism of cell fusion. The present structure of SIV e-gp41 represents one of the largest protein structures determined by NMR to date.

PubMed: 9707417
DOI: 10.1093/emboj/17.16.4572

実験手法

SOLUTION NMR