2EZM

SOLUTION NMR STRUCTURE OF CYANOVIRIN-N, RESTRAINED REGULARIZED MEAN COORDINATES

2EZM の概要

• エントリーDOI: 10.2210/pdb2ezm/pdb
• 分子名称: CYANOVIRIN-N (1 entity in total)
• 機能のキーワード: hiv-inactivating protein
• 由来する生物種: Nostoc ellipsosporum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11022.09

構造登録者

Bewley, C.A.,Gronenborn, A.M.,Clore, G.M. (登録日: 1998-05-06, 公開日: 1999-05-11, 最終更新日: 2024-10-16)

主引用文献

Bewley, C.A.,Gustafson, K.R.,Boyd, M.R.,Covell, D.G.,Bax, A.,Clore, G.M.,Gronenborn, A.M.
Solution structure of cyanovirin-N, a potent HIV-inactivating protein.
Nat.Struct.Biol., 5:571-578, 1998

PubMed Abstract: The solution structure of cyanovirin-N, a potent 11,000 Mr HIV-inactivating protein that binds with high affinity and specificity to the HIV surface envelope protein gp120, has been solved by nuclear magnetic resonance spectroscopy, including extensive use of dipolar couplings which provide a priori long range structural information. Cyanovirin-N is an elongated, largely beta-sheet protein that displays internal two-fold pseudosymmetry. The two sequence repeats (residues 1-50 and 51-101) share 32% sequence identity and superimpose with a backbone atomic root-mean-square difference of 1.3 A. The two repeats, however, do not form separate domains since the overall fold is dependent on numerous contacts between them. Rather, two symmetrically related domains are formed by strand exchange between the two repeats. Analysis of surface hydrophobic clusters suggests the location of potential binding sites for protein-protein interactions.

PubMed: 9665171
DOI: 10.1038/828

実験手法

SOLUTION NMR