2EVZ

Structure of RNA Binding Domains 3 and 4 of Polypyrimidine Tract Binding Protein

Summary for 2EVZ

• Entry DOI: 10.2210/pdb2evz/pdb
• Descriptor: Polypyrimidine tract-binding protein 1 (1 entity in total)
• Functional Keywords: alpha-beta sandwich, rna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P26599
• Total number of polymer chains: 1
• Total formula weight: 25266.87

Authors

Allain, F.H.,Auweter, S.D. (deposition date: 2005-11-01, release date: 2006-01-17, Last modification date: 2024-05-22)

Primary citation

Vitali, F.,Henning, A.,Oberstrass, F.C.,Hargous, Y.,Auweter, S.D.,Erat, M.,Allain, F.H.
Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling
Embo J., 25:150-162, 2006

PubMed Abstract: The polypyrimidine tract binding protein (PTB) is a 58 kDa protein involved in many aspects of RNA metabolism. In this study, we focused our attention on the structure of the two C-terminal RNA recognition motifs (RRM3 and RRM4) of PTB. In a previous study, it was found that the two RRMs are independent in the free state. We recently determined the structure of the same fragment in complex with RNA and found that the two RRMs interact extensively. This difference made us re-evaluate in detail the free protein structure and in particular the interdomain interface. We used a combination of NMR spectroscopy and segmental isotopic labeling to unambiguously study and characterize the interdomain interactions. An improved segmental isotopic labeling protocol was used, enabling us to unambiguously identify 130 interdomain NOEs between the two RRMs and to calculate a very precise structure. The structure reveals a large interdomain interface, resulting in a very unusual positioning of the two RRM domains relative to one another.

PubMed: 16362043
DOI: 10.1038/sj.emboj.7600911

Experimental method

SOLUTION NMR