2ES6

Structure of the SAM domain of Vts1p

2ES6 の概要

• エントリーDOI: 10.2210/pdb2es6/pdb
• 分子名称: Vts1p (1 entity in total)
• 機能のキーワード: sam domain, protein structure, gene regulation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11561.48

構造登録者

Allain, F.H.T. (登録日: 2005-10-25, 公開日: 2006-01-24, 最終更新日: 2024-05-22)

主引用文献

Oberstrass, F.C.,Lee, A.,Stefl, R.,Janis, M.,Chanfreau, G.,Allain, F.H.
Shape-specific recognition in the structure of the Vts1p SAM domain with RNA.
Nat.Struct.Mol.Biol., 13:160-167, 2006

PubMed Abstract: Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE.

PubMed: 16429156
DOI: 10.1038/nsmb1038

実験手法

SOLUTION NMR