2ES5
Structure of the SRE RNA
Summary for 2ES5
| Entry DOI | 10.2210/pdb2es5/pdb |
| Descriptor | 5'-R(*GP*GP*AP*GP*AP*GP*GP*CP*UP*CP*UP*GP*GP*CP*AP*GP*CP*UP*UP*UP*UP*CP*C)-3' (1 entity in total) |
| Functional Keywords | rna structure, stem-loop, pentaloop, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 7372.39 |
| Authors | Allain, F.H.T. (deposition date: 2005-10-25, release date: 2006-01-24, Last modification date: 2024-05-22) |
| Primary citation | Oberstrass, F.C.,Lee, A.,Stefl, R.,Janis, M.,Chanfreau, G.,Allain, F.H. Shape-specific recognition in the structure of the Vts1p SAM domain with RNA. Nat.Struct.Mol.Biol., 13:160-167, 2006 Cited by PubMed Abstract: Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE. PubMed: 16429156DOI: 10.1038/nsmb1038 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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