2ERS

Solution structure of the Interleukin-15 receptor sushi domain

2ERS の概要

• エントリーDOI: 10.2210/pdb2ers/pdb
• NMR情報: BMRB: 6882
• 分子名称: Interleukin-15 receptor alpha chain (1 entity in total)
• 機能のキーワード: sushi domain, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein. Isoform 5: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Isoform 6: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Isoform 7: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Isoform 8: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Soluble interleukin-15 receptor subunit alpha: Secreted, extracellular space: Q13261
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7473.59

構造登録者

Lorenzen, I.,Dingley, A.J.,Grotzinger, J. (登録日: 2005-10-25, 公開日: 2006-02-07, 最終更新日: 2024-10-16)

主引用文献

Lorenzen, I.,Dingley, A.J.,Jacques, Y.,Grotzinger, J.
The structure of the interleukin-15 alpha receptor and its implications for ligand binding.
J.Biol.Chem., 281:6642-6647, 2006

PubMed Abstract: Interleukin (IL)-15 is a member of the small four alpha-helix bundle family of cytokines. IL-15 was discovered by its ability to mimic IL-2-mediated T-cell proliferation. Both cytokines share the beta and gamma receptor chains of the IL-2 receptor for signal transduction. However, in addition, they target specific alpha chain receptors IL-15Ralpha and IL-2Ralpha, respectively. The exceptionally high affinity binding of IL-15 to IL-15Ralpha is mediated by its sushi domain. Here we present the solution structure of the IL-15Ralpha sushi domain solved by NMR spectroscopy and a model of its complex with IL-15. The model shows that, rather than the familiar hydrophobic forces dominating the interaction interface between cytokines and their cognate receptors, the interaction between the IL-15 and IL-15Ralpha complex involves a large network of ionic interactions. This type of interaction explains the exceptionally high affinity of the IL-15.IL-15Ralpha complex, which is essential for the biological effects of this important cytokine and which is not observed in other cytokine/cytokine receptor complexes.

PubMed: 16377614
DOI: 10.1074/jbc.M513118200

実験手法

SOLUTION NMR