2ERR

NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU

2ERR の概要

• エントリーDOI: 10.2210/pdb2err/pdb
• NMR情報: BMRB: 6895
• 分子名称: UGCAUGU, Ataxin-2-binding protein 1 (2 entities in total)
• 機能のキーワード: protein-rna complex, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus (By similarity): Q9NWB1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14598.32

構造登録者

Allain, F.H.,Auweter, S.D. (登録日: 2005-10-25, 公開日: 2006-01-10, 最終更新日: 2024-05-22)

主引用文献

Auweter, S.D.,Fasan, R.,Reymond, L.,Underwood, J.G.,Black, D.L.,Pitsch, S.,Allain, F.H.
Molecular basis of RNA recognition by the human alternative splicing factor Fox-1.
Embo J., 25:163-173, 2006

PubMed Abstract: The Fox-1 protein regulates alternative splicing of tissue-specific exons by binding to GCAUG elements. Here, we report the solution structure of the Fox-1 RNA binding domain (RBD) in complex with UGCAUGU. The last three nucleotides, UGU, are recognized in a canonical way by the four-stranded beta-sheet of the RBD. In contrast, the first four nucleotides, UGCA, are bound by two loops of the protein in an unprecedented manner. Nucleotides U1, G2, and C3 are wrapped around a single phenylalanine, while G2 and A4 form a base-pair. This novel RNA binding site is independent from the beta-sheet binding interface. Surface plasmon resonance analyses were used to quantify the energetic contributions of electrostatic and hydrogen bond interactions to complex formation and support our structural findings. These results demonstrate the unusual molecular mechanism of sequence-specific RNA recognition by Fox-1, which is exceptional in its high affinity for a defined but short sequence element.

PubMed: 16362037
DOI: 10.1038/sj.emboj.7600918

実験手法

SOLUTION NMR