2ERF

Crystal Structure of the Thrombospondin-1 N-terminal Domain at 1.45A Resolution

2ERF の概要

• エントリーDOI: 10.2210/pdb2erf/pdb
• 関連するPDBエントリー: 1Z78 1ZA4
• 分子名称: Thrombospondin-1 (2 entities in total)
• 機能のキーワード: thrombospondin-1, tsp-1, n-terminal tspn, hbd, sugar binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22901.03

構造登録者

Tan, K.,Wang, J.,Lawler, J. (登録日: 2005-10-24, 公開日: 2006-01-17, 最終更新日: 2024-11-06)

主引用文献

Tan, K.,Duquette, M.,Liu, J.H.,Zhang, R.,Joachimiak, A.,Wang, J.H.,Lawler, J.
The structures of the thrombospondin-1 N-terminal domain and its complex with a synthetic pentameric heparin.
Structure, 14:33-42, 2006

PubMed Abstract: The N-terminal domain of thrombospondin-1 (TSPN-1) mediates the protein's interaction with (1) glycosaminoglycans, calreticulin, and integrins during cellular adhesion, (2) low-density lipoprotein receptor-related protein during uptake and clearance, and (3) fibrinogen during platelet aggregation. The crystal structure of TSPN-1 to 1.8 A resolution is a beta sandwich with 13 antiparallel beta strands and 1 irregular strand-like segment. Unique structural features of the N- and C-terminal regions, and the disulfide bond location, distinguish TSPN-1 from the laminin G domain and other concanavalin A-like lectins/glucanases superfamily members. The crystal structure of the complex of TSPN-1 with heparin indicates that residues R29, R42, and R77 in an extensive positively charged patch at the bottom of the domain specifically associate with the sulfate groups of heparin. The TSPN-1 structure and identified adjacent linker region provide a structural framework for the analysis of the TSPN domain of various molecules, including TSPs, NELLs, many collagens, TSPEAR, and kielin.

PubMed: 16407063
DOI: 10.1016/j.str.2005.09.017

実験手法

X-RAY DIFFRACTION (1.45 Å)