2EFK

Crystal structure of the EFC domain of Cdc42-interacting protein 4

Summary for 2EFK

• Entry DOI: 10.2210/pdb2efk/pdb
• Descriptor: Cdc42-interacting protein 4 (2 entities in total)
• Functional Keywords: efc domain, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, endocytosis-exocytosis complex, endocytosis/exocytosis
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm, cytoskeleton. Isoform 5: Cytoplasm, perinuclear region: Q15642
• Total number of polymer chains: 1
• Total formula weight: 34970.08

Authors

Shimada, A.,Niwa, H.,Chen, L.,Liu, Z.-J.,Wang, B.-C.,Terada, T.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2007-02-23, release date: 2007-05-29, Last modification date: 2024-10-23)

Primary citation

Shimada, A.,Niwa, H.,Tsujita, K.,Suetsugu, S.,Nitta, K.,Hanawa-Suetsugu, K.,Akasaka, R.,Nishino, Y.,Toyama, M.,Chen, L.,Liu, Z.-J.,Wang, B.-C.,Yamamoto, M.,Terada, T.,Miyazawa, A.,Tanaka, A.,Sugano, S.,Shirouzu, M.,Nagayama, K.,Takenawa, T.,Yokoyama, S.
Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis
Cell(Cambridge,Mass.), 129:761-772, 2007

PubMed Abstract: Pombe Cdc15 homology (PCH) proteins play an important role in a variety of actin-based processes, including clathrin-mediated endocytosis (CME). The defining feature of the PCH proteins is an evolutionarily conserved EFC/F-BAR domain for membrane association and tubulation. In the present study, we solved the crystal structures of the EFC domains of human FBP17 and CIP4. The structures revealed a gently curved helical-bundle dimer of approximately 220 A in length, which forms filaments through end-to-end interactions in the crystals. The curved EFC dimer fits a tubular membrane with an approximately 600 A diameter. We subsequently proposed a model in which the curved EFC filament drives tubulation. In fact, striation of tubular membranes was observed by phase-contrast cryo-transmission electron microscopy, and mutations that impaired filament formation also impaired membrane tubulation and cell membrane invagination. Furthermore, FBP17 is recruited to clathrin-coated pits in the late stage of CME, indicating its physiological role.

PubMed: 17512409
DOI: 10.1016/j.cell.2007.03.040

Experimental method

X-RAY DIFFRACTION (2.3 Å)