2E85

Crystal Structure of the Hydrogenase 3 Maturation protease

2E85 の概要

• エントリーDOI: 10.2210/pdb2e85/pdb
• 分子名称: Hydrogenase 3 maturation protease, CALCIUM ION (3 entities in total)
• 機能のキーワード: hydrogenase, maturation, protease, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34767.67

構造登録者

Tanaka, T.,Kumarevel, T.S.,Shinkai, A.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2007-01-18, 公開日: 2008-01-22, 最終更新日: 2024-05-29)

主引用文献

Kumarevel, T.,Tanaka, T.,Bessho, Y.,Shinkai, A.,Yokoyama, S.
Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli
Biochem.Biophys.Res.Commun., 389:310-314, 2009

PubMed Abstract: The maturation of [NiFe]-hydrogenases is a catalyzed process involving the activities of at least seven proteins. The last step consists of the endoproteolytic cleavage of the precursor of the large subunit, after the [NiFe]-metal center has been assembled. The HycI endopeptidase is involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3 from Escherichia coli. Although HycI has been well characterized biochemically, the crystallization of the protein has been quite challenging. Here, we present the crystal structure of HycI at 1.70 A resolution. The crystal structure resembles the recently reported solution structure (NMR) of the same protein and the holo-HyPD structure of the same family, but a significant conformational change is observed at the L5 loop, as compared with the solution structures of HycI and HyPD. In our crystal structure, three specific metal binding sites (Ca1-3) were identified and these metal ions are possibly involved in the C-terminal cleavage of HycE.

PubMed: 19720045
DOI: 10.1016/j.bbrc.2009.08.135

実験手法

X-RAY DIFFRACTION (1.7 Å)