2E7L

Structure of a high-affinity mutant of the 2C TCR in complex with Ld/QL9

2E7L の概要

• エントリーDOI: 10.2210/pdb2e7l/pdb
• 関連するPDBエントリー: 2OI9
• 分子名称: Cytotoxic Tcell receptor, Beta-chain, H-2 class I histocompatibility antigen, L-D alpha chain, ... (5 entities in total)
• 機能のキーワード: tcr, mhc, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01897
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 96470.72

構造登録者

Garcia, K.C.,Colf, L.A. (登録日: 2007-01-11, 公開日: 2007-04-24, 最終更新日: 2024-10-16)

主引用文献

Colf, L.A.,Bankovich, A.J.,Hanick, N.A.,Bowerman, N.A.,Jones, L.L.,Kranz, D.M.,Garcia, K.C.
How a single T cell receptor recognizes both self and foreign MHC.
Cell(Cambridge,Mass.), 129:135-146, 2007

PubMed Abstract: alphabeta T cell receptors (TCRs) can crossreact with both self- and foreign- major histocompatibility complex (MHC) proteins in an enigmatic phenomenon termed alloreactivity. Here we present the 2.35 A structure of the 2C TCR complexed with its foreign ligand H-2L(d)-QL9. Surprisingly, we find that this TCR utilizes a different strategy to engage the foreign pMHC in comparison to the manner in which it recognizes a self ligand H-2K(b)-dEV8. 2C engages both shared and polymorphic residues on L(d) and K(b), as well as the unrelated QL9 and dEV8 peptide antigens, in unique pair-wise contacts, resulting in greater structural complementarity with the L(d)-QL9 complex. In the structure of an engineered, high-affinity 2C TCR variant bound to H-2L(d)-QL9, the "wild-type" TCR-MHC binding orientation persists despite modified TCR-CDR3alpha interactions with peptide. Thus, a single TCR recognizes two globally similar, but distinct ligands by divergent mechanisms, indicating that receptor-ligand crossreactivity can occur in the absence of molecular mimicry.

PubMed: 17418792
DOI: 10.1016/j.cell.2007.01.048

実験手法

X-RAY DIFFRACTION (2.5 Å)