2E61

Solution structure of the zf-CW domain in zinc finger CW-type PWWP domain protein 1

2E61 の概要

• エントリーDOI: 10.2210/pdb2e61/pdb
• NMR情報: BMRB: 11358,11359,11360,11361,11362
• 分子名称: Zinc finger CW-type PWWP domain protein 1, ZINC ION (2 entities in total)
• 機能のキーワード: zf-cw domain, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, unknown function
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7713.76

構造登録者

He, F.,Muto, Y.,Inoue, M.,Kigawa, T.,Shirouzu, M.,Terada, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2006-12-25, 公開日: 2007-06-26, 最終更新日: 2024-05-01)

主引用文献

He, F.,Umehara, T.,Saito, K.,Harada, T.,Watanabe, S.,Yabuki, T.,Kigawa, T.,Takahashi, M.,Kuwasako, K.,Tsuda, K.,Matsuda, T.,Aoki, M.,Seki, E.,Kobayashi, N.,Guntert, P.,Yokoyama, S.,Muto, Y.
Structural insight into the zinc finger CW domain as a histone modification reader
Structure, 18:1127-1139, 2010

PubMed Abstract: The zinc finger CW (zf-CW) domain is a motif of about 60 residues that is frequently found in proteins involved in epigenetic regulation. Here, we determined the NMR solution structure of the zf-CW domain of the human zf-CW and PWWP domain containing protein 1 (ZCWPW1). The zf-CW domain adopts a new fold in which a zinc ion is coordinated tetrahedrally by four conserved Cys ligand residues. The tertiary structure of the zf-CW domain partially resembles that adopted by the plant homeo domain (PHD) finger bound to the histone tail, suggesting that the zf-CW domain and the PHD finger have similar functions. The solution structure of the complex of the zf-CW domain with the histone H3 tail peptide (1-10) with trimethylated K4 clarified its binding mode. Our structural and biochemical studies have identified the zf-CW domain as a member of the histone modification reader modules for epigenetic regulation.

PubMed: 20826339
DOI: 10.1016/j.str.2010.06.012

実験手法

SOLUTION NMR