2E5M
Crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain 7
Summary for 2E5M
| Entry DOI | 10.2210/pdb2e5m/pdb |
| Related | 2E0C |
| Descriptor | 409aa long hypothetical NADP-dependent isocitrate dehydrogenase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total) |
| Functional Keywords | nadp-dependent isocitrate dehydrogenase, oxidoreductase |
| Biological source | Sulfolobus tokodaii str. 7 |
| Total number of polymer chains | 2 |
| Total formula weight | 94604.55 |
| Authors | Kouyama, T.,Kondo, H. (deposition date: 2006-12-22, release date: 2008-01-15, Last modification date: 2023-10-25) |
| Primary citation | Kondo, H.,Murakami, M. Crystal Structures of the Putative Isocitrate Dehydrogenase fromSulfolobus tokodaiiStrain 7 in the Apo and NADP+-Bound Forms. Archaea, 2018:7571984-7571984, 2018 Cited by PubMed Abstract: Isocitrate dehydrogenase is a catabolic enzyme that acts during the third step of the tricarboxylic acid cycle. The hypothetical protein ST2166 from the archaeon was isolated and crystallized. It shares high primary structure homology with prokaryotic NADP-dependent IDHs, suggesting that these enzymes share a common enzymatic mechanism. The crystal structure of ST2166 was determined at 2.0 Å resolution in the apo form, and then the structure of the crystal soaked with NADP was also determined at 2.4 Å resolution, which contained NADP bound at the putative active site. Comparisons between the structures of apo and NADP-bound forms and NADP-IDHs from other prokaryotes suggest that prokaryotic NADP-IDHs recognize their cofactors using conserved Lys335, Tyr336, and Arg386 in ST2166 at the opening cleft before the domain closure. PubMed: 30662370DOI: 10.1155/2018/7571984 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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