2E33

Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase

2E33 の概要

• エントリーDOI: 10.2210/pdb2e33/pdb
• 関連するPDBエントリー: 2E31 2E32
• 分子名称: F-box only protein 2, Ribonuclease pancreatic, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: ubiquitin, scf, fbs1, rnaseb, ligase-hydrolase complex, ligase/hydrolase
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37212.78

構造登録者

Mizushima, T.,Yoshida, Y.,Kumanomidou, T.,Hasegawa, Y.,Yamane, T.,Tanaka, K. (登録日: 2006-11-20, 公開日: 2007-03-20, 最終更新日: 2024-11-20)

主引用文献

Mizushima, T.,Yoshida, Y.,Kumanomidou, T.,Hasegawa, Y.,Suzuki, A.,Yamane, T.,Tanaka, K.
Structural basis for the selection of glycosylated substrates by SCFFbs1 ubiquitin ligase
Proc.Natl.Acad.Sci.Usa, 104:5777-5781, 2007

PubMed Abstract: The ubiquitin ligase complex SCF(Fbs1), which contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway. In SCF ubiquitin ligases, a diverse array of F-box proteins confers substrate specificity. Fbs1/Fbx2, a member of the F-box protein family, recognizes high-mannose oligosaccharides. To elucidate the structural basis of SCF(Fbs1) function, we determined the crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain (SBD) of the Fbs1-glycoprotein complex. The mechanistic model indicated by the structures appears to be well conserved among the SCF ubiquitin ligases. The structure of the SBD-glycoprotein complex indicates that the SBD primarily recognizes Man(3)GlcNAc(2), thereby explaining the broad activity of the enzyme against various glycoproteins. Comparison of two crystal structures of the Skp1-Fbs1 complex revealed the relative motion of a linker segment between the F-box and the SBD domains, which might underlie the ability of the complex to recognize different acceptor lysine residues for ubiquitination.

PubMed: 17389369
DOI: 10.1073/pnas.0610312104

実験手法

X-RAY DIFFRACTION (2.7 Å)