2E1X

NMR structure of the HIV-2 nucleocapsid protein

2E1X の概要

• エントリーDOI: 10.2210/pdb2e1x/pdb
• 分子名称: Gag-Pol polyprotein (Pr160Gag-Pol), ZINC ION (2 entities in total)
• 機能のキーワード: nucleocapsid protein, hiv-2, rna recognition, zinc finger, viral protein
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P18042
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3085.99

構造登録者

Matsui, T.,Kodera, Y.,Miyauchi, E.,Tanaka, H.,Endoh, H.,Komatsu, H.,Tanaka, T.,Kohno, T.,Maeda, T. (登録日: 2006-11-03, 公開日: 2007-06-05, 最終更新日: 2024-11-13)

主引用文献

Matsui, T.,Kodera, Y.,Miyauchi, E.,Tanaka, H.,Endoh, H.,Komatsu, H.,Tanaka, T.,Kohno, T.,Maeda, T.
Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality
Biochem.Biophys.Res.Commun., 358:673-678, 2007

PubMed Abstract: Nucleocapsid protein of HIV, containing two CCHC-type zinc fingers connected by a linker, is a multi-functional protein involved in many critical steps of the HIV life cycle. Several in vitro investigations demonstrated that the reactivities of the first zinc finger flanked by the linker of HIV-1 NCp7 and HIV-2 NCp8 were essential for binding to viral RNA, however, that of the second zinc finger flanked by the linker of NCp7 was very weak and non-specific, whereas the part of NCp8 called NCp8-f2, interacted strongly and specifically with viral RNA. In this study, the three-dimensional structure of NCp8-f2 was determined for the first time. Furthermore, we established that NCp8-f2 specifically binds to the stem-loop SD in viral RNA, and that the hydrophobic cleft and the basic residues close to the cleft were essential for specific binding to SD. We discuss the functional significance of NCp8-f2 for NCp8 being a multi-functional protein.

PubMed: 17511966
DOI: 10.1016/j.bbrc.2007.04.141

実験手法

SOLUTION NMR