2E1U

Crystal structure of Dendranthema morifolium DmAT

2E1U の概要

• エントリーDOI: 10.2210/pdb2e1u/pdb
• 関連するPDBエントリー: 2E1T 2E1V
• 分子名称: acyl transferase (2 entities in total)
• 機能のキーワード: bahd superfamily, acyl transferase, dendranthema morifolium, dmat, transferase
• 由来する生物種: Chrysanthemum x morifolium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101720.45

構造登録者

Unno, H.,Ichimaida, F.,Kusunoki, M.,Nakayama, T. (登録日: 2006-10-28, 公開日: 2007-04-10, 最終更新日: 2024-10-23)

主引用文献

Unno, H.,Ichimaida, F.,Suzuki, H.,Takahashi, S.,Tanaka, Y.,Saito, A.,Nishino, T.,Kusunoki, M.,Nakayama, T.
Structural and Mutational Studies of Anthocyanin Malonyltransferases Establish the Features of BAHD Enzyme Catalysis
J.Biol.Chem., 282:15812-15822, 2007

PubMed Abstract: The BAHD family is a class of acyl-CoA-dependent acyltransferases that are involved in plant secondary metabolism and show a diverse range of specificities for acyl acceptors. Anthocyanin acyltransferases make up an important class of the BAHD family and catalyze the acylation of anthocyanins that are responsible for most of the red-to-blue colors of flowers. Here, we describe crystallographic and mutational studies of three similar anthocyanin malonyltransferases from red chrysanthemum petals: anthocyanidin 3-O-glucoside-6''-O-malonyltransferase (Dm3MaT1), anthocyanidin 3-O-glucoside-3'', 6''-O-dimalonyltransferase (Dm3MaT2), and a homolog (Dm3MaT3). Mutational analyses revealed that seven amino acid residues in the N- and C-terminal regions are important for the differential acyl-acceptor specificity between Dm3MaT1 and Dm3MaT2. Crystallographic studies of Dm3MaT3 provided the first structure of a BAHD member, complexed with acyl-CoA, showing the detailed interactions between the enzyme and acyl-CoA molecules. The structure, combined with the results of mutational analyses, allowed us to identify the acyl-acceptor binding site of anthocyanin malonyltransferases, which is structurally different from the corresponding portion of vinorine synthase, another BAHD member, thus permitting the diversity of the acyl-acceptor specificity of BAHD family to be understood.

PubMed: 17383962
DOI: 10.1074/jbc.M700638200

実験手法

X-RAY DIFFRACTION (2.2 Å)