2DYD

Solution structure of the PABC domain from Triticum aevestium poly(A)-binding protein

2DYD の概要

• エントリーDOI: 10.2210/pdb2dyd/pdb
• 分子名称: Poly(A)-binding protein (1 entity in total)
• 機能のキーワード: alpha helical protein, rna binding protein
• 由来する生物種: Triticum aestivum (bread wheat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9126.40

構造登録者

Siddiqui, N. (登録日: 2006-09-10, 公開日: 2007-05-22, 最終更新日: 2024-05-29)

主引用文献

Siddiqui, N.,Osborne, M.J.,Gallie, D.R.,Gehring, K.
Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants
Biochemistry, 46:4221-4231, 2007

PubMed Abstract: In animals, the PABC domain from poly (A)-binding protein recruits proteins containing a specific interacting motif (PAM-2) to the mRNP complex. These proteins include Paip1, Paip2, and eukaryotic release factor 3 (eRF3), all of which regulate PABP function in translation. The following reports the solution structure of PABC from Triticum avestium (wheat) poly (A)-binding protein determined by NMR spectroscopy. Wheat PABC (wPABC) is an alpha-helical protein domain, which displays a fold highly similar to the human PABC domain and contains a PAM-2 peptide binding site. Through a bioinformatics search, several plant proteins containing a PAM-2 site were identified including the early response to dehydration protein (ERD-15), which was previously shown to regulate PABP-dependent translation. The plant PAM-2 proteins contain a variety of conserved sequences including a PABP-interacting 1 motif (PAM-1), RNA binding domains, an SMR endonuclease domain, and a poly (A)-nuclease regulatory domain, all of which suggest a function in either translation or mRNA metabolism. The proteins identified are well conserved throughout plant species but have no sequence homologues in metazoans. We show that wPABC binds to the plant PAM-2 motif with high affinity through a conserved mechanism. Overall, our results suggest that plant species have evolved a distinct regulatory mechanism involving novel PABP binding partners.

PubMed: 17358048
DOI: 10.1021/bi061986d

実験手法

SOLUTION NMR