2DUU

Crystal Structure of apo-form of NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase from Synechococcus Sp.

2DUU の概要

• エントリーDOI: 10.2210/pdb2duu/pdb
• 関連するPDBエントリー: 2D2I
• 分子名称: Glyceraldehyde 3-phosphate dehydrogenase, SULFATE ION (3 entities in total)
• 機能のキーワード: glyceraldehyde 3-phosphate dehydrogenase, rossmann fold, apo-form, oxidoreductase
• 由来する生物種: Synechococcus elongatus PCC 7942
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 248879.79

構造登録者

Kitatani, T.,Nakamura, Y.,Wada, K.,Kinoshita, T.,Tamoi, M.,Shigeoka, S.,Tada, T. (登録日: 2006-07-27, 公開日: 2006-09-05, 最終更新日: 2023-10-25)

主引用文献

Kitatani, T.,Nakamura, Y.,Wada, K.,Kinoshita, T.,Tamoi, M.,Shigeoka, S.,Tada, T.
Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942
Acta Crystallogr.,Sect.F, 62:727-730, 2006

PubMed Abstract: The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate dehydrogenase (apo-GAPDH) from Synechococcus PCC 7942 is reported. The crystal structure was solved by molecular replacement and refined to an R of 21.7% and R(free) of 27.5% at 2.9 angstroms resolution. The structural features of apo-GAPDH are as follows. The S-loop has an extremely flexible conformation and the sulfate ion is only taken into the classical P(i) site. A structural comparison with holo-GAPDHs indicated that the S-loop fixation is essential in the discrimination of NADP and NAD molecules.

PubMed: 16880542
DOI: 10.1107/S1744309106027916

実験手法

X-RAY DIFFRACTION (2.9 Å)