2DSK

Crystal structure of catalytic domain of hyperthermophilic chitinase from Pyrococcus furiosus

2DSK の概要

• エントリーDOI: 10.2210/pdb2dsk/pdb
• 分子名称: chitinase, GLYCEROL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: chitinase, catalytic domain, active domain, crystalline chitin, tim-barrel, hydrolase
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70177.08

構造登録者

Nakamura, T.,Mine, S.,Hagihara, Y.,Ishikawa, K.,Uegaki, K. (登録日: 2006-06-30, 公開日: 2007-02-06, 最終更新日: 2024-03-13)

主引用文献

Nakamura, T.,Mine, S.,Hagihara, Y.,Ishikawa, K.,Uegaki, K.
Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus
ACTA CRYSTALLOGR.,SECT.F, 63:7-11, 2007

PubMed Abstract: The crystal structure of the catalytic domain of a chitinase from the hyperthermophilic archaeon Pyrococcus furiosus (AD2(PF-ChiA)) has been determined at 1.5 A resolution. This is the first structure of the catalytic domain of an archaeal chitinase. The overall structure of AD2(PF-ChiA) is a TIM-barrel fold with a tunnel-like active site that is a common feature of family 18 chitinases. Although the catalytic residues (Asp522, Asp524 and Glu526) are conserved, comparison of the conserved residues and structures with those of other homologous chitinases indicates that the catalytic mechanism of PF-ChiA is different from that of family 18 chitinases.

PubMed: 17183162
DOI: 10.1107/S1744309106051773

実験手法

X-RAY DIFFRACTION (1.5 Å)