2DPF

Crystal Structure of curculin1 homodimer

2DPF の概要

• エントリーDOI: 10.2210/pdb2dpf/pdb
• 分子名称: Curculin, SULFATE ION (3 entities in total)
• 機能のキーワード: sweet taste, taste modifying, plant protein
• 由来する生物種: Curculigo latifolia (lumbah)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 51185.25

構造登録者

Kurimoto, E.,Suzuki, M.,Amemiya, E.,Yamaguchi, Y.,Nirasawa, S.,Shimba, N.,Xu, N.,Kashiwagi, T.,Kawai, M.,Suzuki, E.,Kato, K. (登録日: 2006-05-11, 公開日: 2007-05-15, 最終更新日: 2024-10-16)

主引用文献

Kurimoto, E.,Suzuki, M.,Amemiya, E.,Yamaguchi, Y.,Nirasawa, S.,Shimba, N.,Xu, N.,Kashiwagi, T.,Kawai, M.,Suzuki, E.,Kato, K.
Curculin Exhibits Sweet-tasting and Taste-modifying Activities through Its Distinct Molecular Surfaces.
J.Biol.Chem., 282:33252-33256, 2007

PubMed Abstract: Curculin isolated from Curculigo latifolia, a plant grown in Malaysia, has an intriguing property of modifying sour taste into sweet taste. In addition to this taste-modifying activity, curculin itself elicits a sweet taste. Although these activities have been attributed to the heterodimeric isoform and not homodimers of curculin, the underlying mechanisms for the dual action of this protein have been largely unknown. To identify critical sites for these activities, we performed a mutational and structural study of recombinant curculin. Based on the comparison of crystal structures of curculin homo- and heterodimers, a series of mutants was designed and subjected to tasting assays. Mapping of amino acid residues on the three-dimensional structure according to their mutational effects revealed that the curculin heterodimer exhibits sweet-tasting and taste-modifying activities through its partially overlapping but distinct molecular surfaces. These findings suggest that the two activities of the curculin heterodimer are expressed through its two different modes of interactions with the T1R2-T1R3 heterodimeric sweet taste receptor.

PubMed: 17895249
DOI: 10.1074/jbc.C700174200

実験手法

X-RAY DIFFRACTION (1.5 Å)