2DOQ

crystal structure of Sfi1p/Cdc31p complex

2DOQ の概要

• エントリーDOI: 10.2210/pdb2doq/pdb
• 分子名称: Cell division control protein 31, SFI1p, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: sfi1p, centrin, cdc31p, spindle pole body, centrosome, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus, nuclear pore complex: P06704; Cytoplasm, cytoskeleton, spindle pole : Q12369
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 68844.34

構造登録者

Li, S.,Sandercock, A.M.,Conduit, P.T.,Robinson, C.V.,Williams, R.L.,Kilmartin, J.V. (登録日: 2006-05-03, 公開日: 2006-06-27, 最終更新日: 2024-10-30)

主引用文献

Li, S.,Sandercock, A.M.,Conduit, P.,Robinson, C.V.,Williams, R.L.,Kilmartin, J.V.
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication.
J.Cell Biol., 173:867-877, 2006

PubMed Abstract: Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.

PubMed: 16785321
DOI: 10.1083/jcb.200603153

実験手法

X-RAY DIFFRACTION (3 Å)