2DOG

Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8

2DOG の概要

• エントリーDOI: 10.2210/pdb2dog/pdb
• NMR情報: BMRB: 10138
• 分子名称: Probable 16S rRNA-processing protein rimM (1 entity in total)
• 機能のキーワード: beta barrel, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, structural genomics, unknown function
• 由来する生物種: Thermus thermophilus
• 細胞内の位置: Cytoplasm (By similarity): Q5SJH5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9493.97

構造登録者

Suzuki, S.,Matsumoto, E.,Tatsuguchi, A.,Kawazoe, M.,Kaminishi, T.,Takemoto, C.,Shirouzu, M.,Muto, Y.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2006-04-28, 公開日: 2007-04-17, 最終更新日: 2024-05-01)

主引用文献

Suzuki, S.,Tatsuguchi, A.,Matsumoto, E.,Kawazoe, M.,Kaminishi, T.,Shirouzu, M.,Muto, Y.,Takemoto, C.,Yokoyama, S.
Structural characterization of the ribosome maturation protein, RimM
J.Bacteriol., 189:6397-6406, 2007

PubMed Abstract: The RimM protein has been implicated in the maturation of the 30S ribosomal subunit. It binds to ribosomal protein S19, located in the head domain of the 30S subunit. Multiple sequence alignments predicted that RimM possesses two domains in its N- and C-terminal regions. In the present study, we have produced Thermus thermophilus RimM in both the full-length form (162 residues) and its N-terminal fragment, spanning residues 1 to 85, as soluble proteins in Escherichia coli and have performed structural analyses by nuclear magnetic resonance spectroscopy. Residues 1 to 80 of the RimM protein fold into a single structural domain adopting a six-stranded beta-barrel fold. On the other hand, the C-terminal region of RimM (residues 81 to 162) is partly folded in solution. Analyses of 1H-15N heteronuclear single quantum correlation spectra revealed that a wide range of residues in the C-terminal region, as well as the residues in the vicinity of a hydrophobic patch in the N-terminal domain, were dramatically affected upon complex formation with ribosomal protein S19.

PubMed: 17616598
DOI: 10.1128/JB.00024-07

実験手法

SOLUTION NMR