2DJY

Solution structure of Smurf2 WW3 domain-Smad7 PY peptide complex

2DJY の概要

• エントリーDOI: 10.2210/pdb2djy/pdb
• 分子名称: Smad ubiquitination regulatory factor 2, Mothers against decapentaplegic homolog 7 (2 entities in total)
• 機能のキーワード: beta sheet, polyproline type ii helix, ppii, ligase-signaling protein complex, ligase/signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q9HAU4 O15105
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6806.46

構造登録者

Chong, P.A.,Lin, H,Wrana, J.L.,Forman-Kay, J.D. (登録日: 2006-04-06, 公開日: 2006-05-02, 最終更新日: 2024-05-29)

主引用文献

Chong, P.A.,Lin, H.,Wrana, J.L.,Forman-Kay, J.D.
An Expanded WW Domain Recognition Motif Revealed by the Interaction between Smad7 and the E3 Ubiquitin Ligase Smurf2.
J.Biol.Chem., 281:17069-17075, 2006

PubMed Abstract: Smurf2 is an E3 ubiquitin ligase that drives degradation of the transforming growth factor-beta receptors and other targets. Recognition of the receptors by Smurf2 is accomplished through an intermediary protein, Smad7. Here we have demonstrated that the WW3 domain of Smurf2 can directly bind to the Smad7 polyproline-tyrosine (PY) motif. Of particular interest, the highly conserved WW domain binding site Trp, which interacts with target PY motifs, is a Phe in the Smurf2 WW3 domain. To examine this interaction, the solution structure of the complex between the Smad7 PY motif region (ELESPPPPYSRYPMD) and the Smurf2 WW3 domain was determined. The structure reveals that, in addition to binding the PY motif, the WW3 domain binds six residues C-terminal to the PY motif (PY-tail). Although the Phe in the WW3 domain binding site decreases affinity relative to the canonical Trp, this is balanced by additional interactions between the PY-tail and the beta1-strand and beta1-beta2 loop of the WW3 domain. The interaction between the Smurf2 WW3 domain and the Smad7 PY motif is the first example of PY motif recognition by a WW domain with a Phe substituted for the binding site Trp. This unusual interaction allows the Smurf2 WW3 domain to recognize a subset of PY motif-containing proteins utilizing an expanded surface to provide specificity.

PubMed: 16641086
DOI: 10.1074/jbc.M601493200

実験手法

SOLUTION NMR