2DI2
NMR structure of the HIV-2 nucleocapsid protein
2DI2 の概要
エントリーDOI | 10.2210/pdb2di2/pdb |
関連するPDBエントリー | 1NC8 |
分子名称 | Nucleocapsid protein p7, ZINC ION (2 entities in total) |
機能のキーワード | nucleocapsid protein, hiv-2, rna recognition, zinc finger, mutant, metal binding protein |
由来する生物種 | synthetic construct |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 3412.31 |
構造登録者 | Matsui, T.,Kodera, Y.,Endoh, H.,Miyauchi, E.,Komatsu, H.,Sato, K.,Tanaka, T.,Kohno, T.,Maeda, T. (登録日: 2006-03-27, 公開日: 2007-03-13, 最終更新日: 2024-05-29) |
主引用文献 | Matsui, T.,Kodera, Y.,Endoh, H.,Miyauchi, E.,Komatsu, H.,Sato, K.,Tanaka, T.,Kohno, T.,Maeda, T. RNA Recognition Mechanism of the Minimal Active Domain of the Human Immunodeficiency Virus Type-2 Nucleocapsid Protein J.Biochem.(Tokyo), 141:269-277, 2007 Cited by PubMed Abstract: NCp8 of HIV-2 contains two CCHC-type zinc fingers connected by a linker, and is involved in many critical steps of the virus life cycle. It was previously shown that the first zinc finger flanked by the linker is the minimal active domain for specific binding to viral RNA. In our previous study, we determined the three-dimensional structure of NCp8-f1, including the minimal active domain, and found that a hydrogen bond between Asn(11) N(delta)H and Arg(27) O stabilized the conformation of the linker in the vicinity of the zinc finger [Kodera et al. (1998) Biochemistry 37, 17704-17713]. In this study, RNA binding activities of NCp8-f1 and three types of its mutant peptides were analysed by native PAGE assay. The activity and three-dimensional structure of NCp8-f1/N11A, in which alanine is substituted for Asn(11) thereby affecting the conformation of the linker, was analyzed and compared with those of NCp8-f1. We demonstrated that the existence of Arg(4) and/or Lys(5) and Arg(26) and/or Arg(27) were necessary for binding RNA. Furthermore, the linker's flexible orientation, which is controlled by the hydrogen bond between Asn(11) N(delta)H and Arg(27) O, appears to be a structural basis for NCp8 existing as a multi-functional protein. PubMed: 17202191DOI: 10.1093/jb/mvm037 主引用文献が同じPDBエントリー |
実験手法 | SOLUTION NMR |
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