2DHC

CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE

2DHC の概要

• エントリーDOI: 10.2210/pdb2dhc/pdb
• 分子名称: HALOALKANE DEHALOGENASE, 1,2-DICHLOROETHANE (3 entities in total)
• 機能のキーワード: dehalogenase
• 由来する生物種: Xanthobacter autotrophicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35274.76

構造登録者

Verschueren, K.H.G.,Dijkstra, B.W. (登録日: 1993-09-08, 公開日: 1994-07-31, 最終更新日: 2024-02-14)

主引用文献

Verschueren, K.H.,Seljee, F.,Rozeboom, H.J.,Kalk, K.H.,Dijkstra, B.W.
Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase.
Nature, 363:693-698, 1993

PubMed Abstract: Crystal structures of haloalkane dehalogenase were determined in the presence of the substrate 1,2-dichloroethane. At pH 5 and 4 degrees C, substrate is bound in the active site without being converted; warming to room temperature causes the substrate's carbon-chlorine bond to be broken, producing a chloride ion with concomitant alkylation of the active-site residue Asp124. At pH 6 and room temperature the alkylated enzyme is hydrolysed by a water molecule activated by the His289-Asp260 pair in the active site. These results show that catalysis by the dehalogenase proceeds by a two-step mechanism involving an ester intermediate covalently bound at Asp124.

PubMed: 8515812
DOI: 10.1038/363693a0

実験手法

X-RAY DIFFRACTION (2.3 Å)