2DGJ

Crystal structure of EbhA (756-1003 domain) from Staphylococcus aureus

2DGJ の概要

• エントリーDOI: 10.2210/pdb2dgj/pdb
• 関連するPDBエントリー: 1xvh
• 分子名称: hypothetical protein ebhA, SULFATE ION, ACETIC ACID, ... (5 entities in total)
• 機能のキーワード: helix-bundle, rod-like structure, adhesin, cell adhesion
• 由来する生物種: Staphylococcus aureus
• 細胞内の位置: Cell membrane; Single-pass membrane protein (Potential): Q931R6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55453.72

構造登録者

Tanaka, Y.,Yao, M.,Kuroda, M.,Watanabe, N.,Ohta, T.,Tanaka, I. (登録日: 2006-03-14, 公開日: 2007-03-20, 最終更新日: 2024-10-23)

主引用文献

Tanaka, Y.,Sakamoto, S.,Kuroda, M.,Goda, S.,Gao, Y.-G.,Tsumoto, K.,Hiragi, Y.,Yao, M.,Watanabe, N.,Ohta, T.,Tanaka, I.
A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus
Structure, 16:488-496, 2008

PubMed Abstract: The 1.1 MDa cell-wall-associated adhesion protein of staphylococci, Ebh, consists of several distinct regions, including a large central region with 52 imperfect repeats of 126 amino acid residues. We investigated the structure of this giant molecule by X-ray crystallography, circular dichroism (CD) spectrometry, and small-angle X-ray scattering (SAXS). The crystal structure of two repeats showed that each repeat consists of two distinct three-helix bundles, and two such repeats are connected along the long axis, resulting in a rod-like structure that is 120 A in length. CD and SAXS analyses of the samples with longer repeats suggested that each repeat has an identical structure, and that such repeats are connected tandemly to form a rod-like structure in solution, the length of which increased proportionately with the number of repeating units. On the basis of these results, it was proposed that Ebh is a 320 nm rod-like molecule with some plasticity at module junctions.

PubMed: 18334223
DOI: 10.1016/j.str.2007.12.018

実験手法

X-RAY DIFFRACTION (2.35 Å)