2DGE

Crystal structure of oxidized cytochrome C6A from Arabidopsis thaliana

Summary for 2DGE

• Entry DOI: 10.2210/pdb2dge/pdb
• Descriptor: Cytochrome c6, ZINC ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• Functional Keywords: cytochrome c6a, electron transfer, heme exposure, arabidopsis thaliana, electron transport
• Biological source: Arabidopsis thaliana (thale cress)
• Cellular location: Plastid, chloroplast thylakoid lumen (Probable): Q93VA3
• Total number of polymer chains: 4
• Total formula weight: 49461.25

Authors

Chida, H.,Yokoyama, T.,Kawai, F.,Nakazawa, A.,Akazaki, H.,Takayama, Y.,Hirano, T.,Suruga, K.,Satoh, T.,Yamada, S.,Kawachi, R.,Unzai, S.,Nishio, T.,Park, S.-Y.,Oku, T. (deposition date: 2006-03-11, release date: 2006-07-04, Last modification date: 2024-10-16)

Primary citation

Chida, H.,Yokoyama, T.,Kawai, F.,Nakazawa, A.,Akazaki, H.,Takayama, Y.,Hirano, T.,Suruga, K.,Satoh, T.,Yamada, S.,Kawachi, R.,Unzai, S.,Nishio, T.,Park, S.-Y.,Oku, T.
Crystal structure of oxidized cytochrome c(6A) from Arabidopsis thaliana
Febs Lett., 580:3763-3768, 2006

PubMed Abstract: Compared with algal and cyanobacterial cytochrome c(6), cytochrome c(6A) from higher plants contains an additional loop of 12 amino acid residues. We have determined the first crystal structure of cytochrome c(6A) from Arabidopsis thaliana at 1.5 Angstrom resolution in order to help elucidate its function. The overall structure of cytochrome c(6A) follows the topology of class I c-type cytochromes in which the heme prosthetic group covalently binds to Cys16 and Cys19, and the iron has octahedral coordination with His20 and Met60 as the axial ligands. Two cysteine residues (Cys67 and Cys73) within the characteristic 12 amino acids loop form a disulfide bond, contributing to the structural stability of cytochrome c(6A). Our model provides a chemical basis for the known low redox potential of cytochrome c(6A) which makes it an unsuitable electron carrier between cytochrome b(6)f and PSI.

PubMed: 16777100
DOI: 10.1016/j.febslet.2006.05.067

Experimental method

X-RAY DIFFRACTION (1.5 Å)