2DGE

Crystal structure of oxidized cytochrome C6A from Arabidopsis thaliana

2DGE の概要

• エントリーDOI: 10.2210/pdb2dge/pdb
• 分子名称: Cytochrome c6, ZINC ION, HEME C, ... (4 entities in total)
• 機能のキーワード: cytochrome c6a, electron transfer, heme exposure, arabidopsis thaliana, electron transport
• 由来する生物種: Arabidopsis thaliana (thale cress)
• 細胞内の位置: Plastid, chloroplast thylakoid lumen (Probable): Q93VA3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49469.32

構造登録者

Chida, H.,Yokoyama, T.,Kawai, F.,Nakazawa, A.,Akazaki, H.,Takayama, Y.,Hirano, T.,Suruga, K.,Satoh, T.,Yamada, S.,Kawachi, R.,Unzai, S.,Nishio, T.,Park, S.-Y.,Oku, T. (登録日: 2006-03-11, 公開日: 2006-07-04, 最終更新日: 2025-12-24)

主引用文献

Chida, H.,Yokoyama, T.,Kawai, F.,Nakazawa, A.,Akazaki, H.,Takayama, Y.,Hirano, T.,Suruga, K.,Satoh, T.,Yamada, S.,Kawachi, R.,Unzai, S.,Nishio, T.,Park, S.-Y.,Oku, T.
Crystal structure of oxidized cytochrome c(6A) from Arabidopsis thaliana
Febs Lett., 580:3763-3768, 2006

PubMed Abstract: Compared with algal and cyanobacterial cytochrome c(6), cytochrome c(6A) from higher plants contains an additional loop of 12 amino acid residues. We have determined the first crystal structure of cytochrome c(6A) from Arabidopsis thaliana at 1.5 Angstrom resolution in order to help elucidate its function. The overall structure of cytochrome c(6A) follows the topology of class I c-type cytochromes in which the heme prosthetic group covalently binds to Cys16 and Cys19, and the iron has octahedral coordination with His20 and Met60 as the axial ligands. Two cysteine residues (Cys67 and Cys73) within the characteristic 12 amino acids loop form a disulfide bond, contributing to the structural stability of cytochrome c(6A). Our model provides a chemical basis for the known low redox potential of cytochrome c(6A) which makes it an unsuitable electron carrier between cytochrome b(6)f and PSI.

PubMed: 16777100
DOI: 10.1016/j.febslet.2006.05.067

実験手法

X-RAY DIFFRACTION (1.5 Å)