2DF4

Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Mn2+

2DF4 の概要

• エントリーDOI: 10.2210/pdb2df4/pdb
• 関連するPDBエントリー: 2F2A 2G5H 2G5I
• 分子名称: Glutamyl-tRNA(Gln) amidotransferase subunit A, Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B, Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C, ... (5 entities in total)
• 機能のキーワード: multi protein complex, ligase
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 119042.65

構造登録者

Nakamura, A.,Yao, M.,Tanaka, I. (登録日: 2006-02-23, 公開日: 2006-07-18, 最終更新日: 2023-10-25)

主引用文献

Nakamura, A.,Yao, M.,Chimnaronk, S.,Sakai, N.,Tanaka, I.
Ammonia channel couples glutaminase with transamidase reactions in GatCAB
Science, 312:1954-1958, 2006

PubMed Abstract: The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln).

PubMed: 16809541
DOI: 10.1126/science.1127156

実験手法

X-RAY DIFFRACTION (3.2 Å)