2DE7

The substrate-bound complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase

2DE7 の概要

• エントリーDOI: 10.2210/pdb2de7/pdb
• 関連するPDBエントリー: 2DE5 2DE6
• 分子名称: terminal oxygenase component of carbazole, ferredoxin component of carbazole, FE (II) ION, ... (6 entities in total)
• 機能のキーワード: electron transfer complex, rieske non-heme iron oxygenase system, terminal oxygenase, rieske-type ferredoxin, carbazole 1, 9a-dioxygenase, oxidoreductase
• 由来する生物種: Janthinobacterium; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 173633.26

構造登録者

Ashikawa, Y.,Nojiri, H. (登録日: 2006-02-08, 公開日: 2006-12-05, 最終更新日: 2023-10-25)

主引用文献

Ashikawa, Y.,Fujimoto, Z.,Noguchi, H.,Habe, H.,Omori, T.,Yamane, H.,Nojiri, H.
Electron Transfer Complex Formation between Oxygenase and Ferredoxin Components in Rieske Nonheme Iron Oxygenase System
Structure, 14:1779-1789, 2006

PubMed Abstract: Carbazole 1,9a-dioxygenase (CARDO), a member of the Rieske nonheme iron oxygenase system (ROS), consists of a terminal oxygenase (CARDO-O) and electron transfer components (ferredoxin [CARDO-F] and ferredoxin reductase [CARDO-R]). We determined the crystal structures of the nonreduced, reduced, and substrate-bound binary complexes of CARDO-O with its electron donor, CARDO-F, at 1.9, 1.8, and 2.0 A resolutions, respectively. These structures provide the first structure-based interpretation of intercomponent electron transfer between two Rieske [2Fe-2S] clusters of ferredoxin and oxygenase in ROS. Three molecules of CARDO-F bind to the subunit boundary of one CARDO-O trimeric molecule, and specific binding created by electrostatic and hydrophobic interactions with conformational changes suitably aligns the two Rieske clusters for electron transfer. Additionally, conformational changes upon binding carbazole resulted in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site.

PubMed: 17161368
DOI: 10.1016/j.str.2006.10.004

実験手法

X-RAY DIFFRACTION (2 Å)