2D6F

Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)

2D6F の概要

• エントリーDOI: 10.2210/pdb2d6f/pdb
• 分子名称: tRNA, Glutamyl-tRNA(Gln) amidotransferase subunit D, Glutamyl-tRNA(Gln) amidotransferase subunit E, ... (5 entities in total)
• 機能のキーワード: ligase, rna, ligase-rna complex, ligase/rna
• 由来する生物種: Methanothermobacter thermautotrophicus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 283160.20

構造登録者

Nureki, O. (登録日: 2005-11-13, 公開日: 2006-07-11, 最終更新日: 2024-03-13)

主引用文献

Oshikane, H.,Sheppard, K.,Fukai, S.,Nakamura, Y.,Ishitani, R.,Numata, T.,Sherrer, R.L.,Feng, L.,Schmitt, E.,Panvert, M.,Blanquet, S.,Mechulam, Y.,Soll, D.,Nureki, O.
Structural basis of RNA-dependent recruitment of glutamine to the genetic code
Science, 312:1950-1954, 2006

PubMed Abstract: Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code.

PubMed: 16809540
DOI: 10.1126/science.1128470

実験手法

X-RAY DIFFRACTION (3.15 Å)