2D5K

Crystal structure of Dps from Staphylococcus aureus

2D5K の概要

• エントリーDOI: 10.2210/pdb2d5k/pdb
• 関連するPDBエントリー: 1DPS 1JI5 1JIG 1N1Q 1QGH 1UMN
• 分子名称: Dps family protein, GLYCEROL (3 entities in total)
• 機能のキーワード: four helix bundle, metal binding protein
• 由来する生物種: Staphylococcus aureus subsp. aureus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 71575.24

構造登録者

Tanaka, Y.,Yao, M.,Watanabe, N.,Tanaka, I. (登録日: 2005-11-02, 公開日: 2006-10-17, 最終更新日: 2023-10-25)

主引用文献

Ushijima, Y.,Ohniwa, R.L.,Maruyama, A.,Saito, S.,Tanaka, Y.,Morikawa, K.
Nucleoid compaction by MrgA(Asp56Ala/Glu60Ala) does not contribute to staphylococcal cell survival against oxidative stress and phagocytic killing by macrophages
FEMS Microbiol. Lett., 360:144-151, 2014

PubMed Abstract: Staphylococcus aureus MrgA (encoded by mrgA) belongs to the Dps family of proteins, which play important roles in coping with various stresses. The staphylococcal mrgA gene is specifically expressed under oxidative stress conditions and is one of the most highly induced genes during phagocytic killing by macrophages. We previously reported that mrgA is essential for oxidative stress resistance, and can cause nucleoid compaction. However, whether nucleoid compaction by itself would contribute to oxidative stress resistance was hard to determine, because Dps family proteins generally have ferroxidase activity to prevent hydroxyl radical formation via the Fenton reaction. In this study, we resolved the crystal structure of MrgA and conducted mutation analysis of Asp56 and Glu60, which are located at the expected ferroxidase centre. In the strain expressing Asp56Ala/Glu60Ala MrgA (termed MrgA*), MrgA* retained dodecamer formation and nucleoid compaction ability. By contrast, the ferroxidase activity of MrgA* decreased by about half. Viability of the mrgA* strain was as low as the mrgA null mutant in oxidative stress and phagocytic killing assays. These results suggest that nucleoid compaction by itself is insufficient for oxidative stress resistance, and Asp56 and Glu60 constitute essential molecular sites in MrgA for oxidative stress resistance and survival against phagocytic killing.

PubMed: 25227518
DOI: 10.1111/1574-6968.12598

実験手法

X-RAY DIFFRACTION (1.85 Å)