2D43

Crystal structure of arabinofuranosidase complexed with arabinotriose

2D43 の概要

• エントリーDOI: 10.2210/pdb2d43/pdb
• 関連するPDBエントリー: 1WD3 1WD4 2D44
• 分子名称: alpha-L-arabinofuranosidase B, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose, ... (4 entities in total)
• 機能のキーワード: arabinotriose complex, hydrolase, structural genomics
• 由来する生物種: Aspergillus kawachii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51814.39

構造登録者

Miyanaga, A.,Koseki, T.,Miwa, Y.,Matsuzawa, H.,Wakagi, T.,Shoun, H.,Fushinobu, S. (登録日: 2005-10-07, 公開日: 2006-09-19, 最終更新日: 2024-10-16)

主引用文献

Miyanaga, A.,Koseki, T.,Miwa, Y.,Mese, Y.,Nakamura, S.,Kuno, A.,Hirabayashi, J.,Matsuzawa, H.,Wakagi, T.,Shoun, H.,Fushinobu, S.
The family 42 carbohydrate-binding module of family 54 alpha-L-arabinofuranosidase specifically binds the arabinofuranose side chain of hemicellulose
Biochem.J., 399:503-511, 2006

PubMed Abstract: Alpha-L-arabinofuranosidase catalyses the hydrolysis of the alpha-1,2-, alpha-1,3-, and alpha-1,5-L-arabinofuranosidic bonds in L-arabinose-containing hemicelluloses such as arabinoxylan. AkAbf54 (the glycoside hydrolase family 54 alpha-L-arabinofuranosidase from Aspergillus kawachii) consists of two domains, a catalytic and an arabinose-binding domain. The latter has been named AkCBM42 [family 42 CBM (carbohydrate-binding module) of AkAbf54] because homologous domains are classified into CBM family 42. In the complex between AkAbf54 and arabinofuranosyl-alpha-1,2-xylobiose, the arabinose moiety occupies the binding pocket of AkCBM42, whereas the xylobiose moiety is exposed to the solvent. AkCBM42 was found to facilitate the hydrolysis of insoluble arabinoxylan, because mutants at the arabinose binding site exhibited markedly decreased activity. The results of binding assays and affinity gel electrophoresis showed that AkCBM42 interacts with arabinose-substituted, but not with unsubstituted, hemicelluloses. Isothermal titration calorimetry and frontal affinity chromatography analyses showed that the association constant of AkCBM42 with the arabinose moiety is approximately 10(3) M(-1). These results indicate that AkCBM42 binds the non-reducing-end arabinofuranosidic moiety of hemicellulose. To our knowledge, this is the first example of a CBM that can specifically recognize the side-chain monosaccharides of branched hemicelluloses.

PubMed: 16846393
DOI: 10.1042/BJ20060567

実験手法

X-RAY DIFFRACTION (2.8 Å)