2D3R

Cratylia folibunda seed lectin at acidic pH

2D3R の概要

• エントリーDOI: 10.2210/pdb2d3r/pdb
• 関連するPDBエントリー: 2D3P
• 分子名称: Lectin alpha chain, CALCIUM ION, MANGANESE (II) ION, ... (4 entities in total)
• 機能のキーワード: lectin plant, sugar binding protein
• 由来する生物種: Cratylia argentea
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101903.80

構造登録者

Del Sol, F.G.,Cavada, B.S.,Calvete, J.J. (登録日: 2005-09-30, 公開日: 2005-11-10, 最終更新日: 2024-03-13)

主引用文献

Del Sol, F.G.,Cavada, B.S.,Calvete, J.J.
Crystal structures of Cratylia floribunda seed lectin at acidic and basic pHs. Insights into the structural basis of the pH-dependent dimer-tetramer transition.
J.Struct.Biol., 158:1-9, 2007

PubMed Abstract: Structural determinants underlaying the pH-dependent dimer-tetramer transition of Diocleinae lectins were investigated from the structures of Cratylia floribunda seed lectin crystallized in conditions where it exist as a dimer (pH 4.6) or as a tetramer (pH 8.5). The acidic (aCFL) and the basic (bCFL) tetramers superimpose with overall r.m.s.d. of 0.53 A, though interdimer contacts are drastically reduced in aCFL, and the r.m.s.d. for the superposition of the 117-120 loops of aCFL vs. the bCFL tetramer is 1.29 A. Our data support the view that His51 plays a role in determining the conformation of the central cavity loops and that interdimer contacts involving ordered loop residues stabilize the canonical, pH-dependent tetramer. In the bCFL tetramer, hydrogen bonds between Asn118 and Thr120 of monomers A and D and residues Ser66, Ser108, Ser110, and Thr49 of the opposite monomer stabilize the canonical, pH-dependent tetrameric lectin structure. In CFL, Asn131 makes intradimer contacts with Asn122 and Ala123. In comparison, His131 in Dioclea grandiflora lectin establishes a network of interdimer interactions bridging the four central loops of the pH-independent tetramer. Our data provide new insights into the participation of specific amino acid residues in the mechanism of the quaternary association of Diocleinae lectins.

PubMed: 17251039
DOI: 10.1016/j.jsb.2006.08.014

実験手法

X-RAY DIFFRACTION (2.9 Å)