2D00

Subunit F of V-type ATPase/synthase

2D00 の概要

• エントリーDOI: 10.2210/pdb2d00/pdb
• 分子名称: V-type ATP synthase subunit F, CALCIUM ION (3 entities in total)
• 機能のキーワード: v-atpase, subunit f, chey, fret, hydrolase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 71432.33

構造登録者

Makyio, H.,Iino, R.,Ikeda, C.,Imamura, H.,Tamakoshi, M.,Iwata, M.,Stock, D.,Bernal, R.A.,Carpenter, E.P.,Yoshida, M.,Yokoyama, K.,Iwata, S. (登録日: 2005-07-21, 公開日: 2005-12-06, 最終更新日: 2024-03-13)

主引用文献

Makyio, H.,Iino, R.,Ikeda, C.,Imamura, H.,Tamakoshi, M.,Iwata, M.,Stock, D.,Bernal, R.A.,Carpenter, E.P.,Yoshida, M.,Yokoyama, K.,Iwata, S.
Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus
Embo J., 24:3974-3983, 2005

PubMed Abstract: The crystal structure of subunit F of vacuole-type ATPase/synthase (prokaryotic V-ATPase) was determined to of 2.2 A resolution. The subunit reveals unexpected structural similarity to the response regulator proteins that include the Escherichia coli chemotaxis response regulator CheY. The structure was successfully placed into the low-resolution EM structure of the prokaryotic holo-V-ATPase at a location indicated by the results of crosslinking experiments. The crystal structure, together with the single-molecule analysis using fluorescence resonance energy transfer, showed that the subunit F exhibits two conformations, a 'retracted' form in the absence and an 'extended' form in the presence of ATP. Our results postulated that the subunit F is a regulatory subunit in the V-ATPase.

PubMed: 16281059
DOI: 10.1038/sj.emboj.7600859

実験手法

X-RAY DIFFRACTION (2.2 Å)