2CYK

ASPECTS OF RECEPTOR BINDING AND SIGNALLING OF INTERLEUKIN-4 INVESTIGATED BY SITE-DIRECTED MUTAGENESIS AND NMR SPECTROSCOPY

2CYK の概要

• エントリーDOI: 10.2210/pdb2cyk/pdb
• 分子名称: INTERLEUKIN-4 (1 entity in total)
• 機能のキーワード: cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P05112
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14989.25

構造登録者

Mueller, T.,Sebald, W.,Oschkinat, H. (登録日: 1994-08-16, 公開日: 1994-12-20, 最終更新日: 2024-10-30)

主引用文献

Muller, T.,Dieckmann, T.,Sebald, W.,Oschkinat, H.
Aspects of receptor binding and signalling of interleukin-4 investigated by site-directed mutagenesis and NMR spectroscopy.
J.Mol.Biol., 237:423-436, 1994

PubMed Abstract: Cytokines are hormones that carry information from cell to cell. This information is read from their surface upon binding to transmembrane receptors and by the subsequent initiation of receptor oligomerization. An influence on this process through mutagenesis on the hormone surface is highly desirable for medical reasons. However, an understanding of hormone-receptor interactions requires insight into the structural changes introduced by the mutations. In this line structural studies on human IL-4 and the medically important IL-4 antagonists Y124D and Y124G are presented. The site around Y124 is an important epitope responsible for the ability of IL-4 to cause a signal in the target cells. It is shown that the local main-chain structure around residue 124 in the variants remains unchanged. A strategy is presented here which allows the study of these types of proteins and their variants by NMR which does not require carbon labelled samples.

PubMed: 8151703
DOI: 10.1006/jmbi.1994.1245

実験手法

SOLUTION NMR