2CXC

Crystal structure of archaeal transcription termination factor NusA

2CXC の概要

• エントリーDOI: 10.2210/pdb2cxc/pdb
• 分子名称: NusA (2 entities in total)
• 機能のキーワード: transcription termination, rna binding protein, archaeal nusa, kh domain, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, transcription
• 由来する生物種: Aeropyrum pernix
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16069.72

構造登録者

Shibata, R.,Bessho, Y.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2005-06-28, 公開日: 2005-12-28, 最終更新日: 2024-03-13)

主引用文献

Shibata, R.,Bessho, Y.,Shinkai, A.,Nishimoto, M.,Fusatomi, E.,Terada, T.,Shirouzu, M.,Yokoyama, S.
Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA
Biochem.Biophys.Res.Commun., 355:122-128, 2007

PubMed Abstract: The transcription factor NusA functions in transcriptional regulation involving termination in bacteria. A NusA homolog consisting of only the two KH domains is widely conserved in archaea, but its function remains unknown. We have found that Aeropyrum pernix NusA strongly binds to a certain CU-rich sequence near a termination signal. Our crystal structure of A. pernix NusA revealed that its spatial arrangement is quite similar to that of the KH domains of bacterial NusA. Thus, we consider archaeal NusA to have retained some functions of bacterial NusA, including the ssRNA-binding ability. Remarkable structural differences between archaeal and bacterial NusA exist at the interface with RNAP, in connection with the different NusA-binding sites around the termination signals. Transcriptional termination in archaea could differ from all of the known bacterial and eukaryal mechanisms, in terms of the combination of a bacterial factor and a eukaryal-type RNAP.

PubMed: 17288993
DOI: 10.1016/j.bbrc.2007.01.119

実験手法

X-RAY DIFFRACTION (2 Å)