2CSX

Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met)

2CSX の概要

• エントリーDOI: 10.2210/pdb2csx/pdb
• 関連するPDBエントリー: 2CT8
• 分子名称: RNA (75-MER), Methionyl-tRNA synthetase (3 entities in total)
• 機能のキーワード: ligase, riken structural genomics/proteomics initiative, rsgi, structural genomics, ligase-rna complex, ligase/rna
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cytoplasm: O67298
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 166406.18

構造登録者

Nakanishi, K.,Ogiso, Y.,Nakama, T.,Fukai, S.,Nureki, O.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2005-05-23, 公開日: 2005-09-20, 最終更新日: 2024-03-13)

主引用文献

Nakanishi, K.,Ogiso, Y.,Nakama, T.,Fukai, S.,Nureki, O.
Structural basis for anticodon recognition by methionyl-tRNA synthetase.
Nat.Struct.Mol.Biol., 12:931-932, 2005

PubMed Abstract: In the 2.7-A resolution crystal structure of methionyl-tRNA synthetase (MetRS) in complex with tRNA(Met) and a methionyl-adenylate analog, the tRNA anticodon loop is distorted to form a triple-base stack comprising C34, A35 and A38. A tryptophan residue stacks on C34 to extend the triple-base stack. In addition, C34 forms Watson-Crick-type hydrogen bonds with Arg357. This structure resolves the longstanding question of how MetRS specifically recognizes tRNA(Met).

PubMed: 16155581
DOI: 10.1038/nsmb988

実験手法

X-RAY DIFFRACTION (2.7 Å)