2CNR

Structural studies on the interaction of ScFAS ACP with ACPS

2CNR の概要

• エントリーDOI: 10.2210/pdb2cnr/pdb
• 分子名称: ACYL CARRIER PROTEIN (1 entity in total)
• 機能のキーワード: acp, fas, polykdetide, phosphopantetheine, lipid transport
• 由来する生物種: STREPTOMYCES COELICOLOR
• 細胞内の位置: Cytoplasm (By similarity): P72393
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8924.97

構造登録者

Pottage, K.,Williams, C.,Crump, M.P. (登録日: 2006-05-23, 公開日: 2007-06-05, 最終更新日: 2024-05-15)

主引用文献

Arthur, C.J.,Williams, C.,Pottage, K.,Ploskon, E.,Findlow, S.C.,Burston, S.G.,Simpson, T.J.,Crump, M.P.,Crosby, J.
Structure and Malonyl Coa-Acp Transacylase Binding of Streptomyces Coelicolor Fatty Acid Synthase Acyl Carrier Protein.
Acs Chem.Biol., 4:625-, 2009

PubMed Abstract: Malonylation of an acyl carrier protein (ACP) by malonyl Coenzyme A-ACP transacylase (MCAT) is fundamental to bacterial fatty acid biosynthesis. Here, we report the structure of the Steptomyces coelicolor (Sc) fatty acid synthase (FAS) ACP and studies of its binding to MCAT. The carrier protein adopts an alpha-helical bundle structure common to other known carrier proteins. The Sc FAS ACP shows close structural homology with other fatty acid ACPs and less similarity with Sc actinorhodin (act) polyketide synthase (PKS) ACP where the orientation of helix I differs. NMR experiments were used to map the binding of ACP to MCAT. This data suggests that Sc FAS ACP interacts with MCAT through the negatively charged helix II of ACP, consistent with proposed models for ACP recognition by other FAS enzymes. Differential roles for residues at the interface are demonstrated using site-directed mutagenesis and in vitro assays. MCAT has been suggested, moreover, to participate in bacterial polyketide synthesis in vivo. We demonstrate that the affinity of the polyketide synthase ACP for MCAT is lower than that of the FAS ACP. Mutagenesis of homologous helix II residues on the polyketide synthase ACP suggests that the PKS ACP may bind to MCAT in a different manner than the FAS counterpart.

PubMed: 19555075
DOI: 10.1021/CB900099E

実験手法

SOLUTION NMR