2CJ5

Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco (pH 5.0)

2CJ5 の概要

• エントリーDOI: 10.2210/pdb2cj5/pdb
• 関連するPDBエントリー: 1RJ1 1RJ4 2CJ4 2CJ6 2CJ7 2CJ8
• 分子名称: INVERTASE INHIBITOR, ACETATE ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: inhibitor, protein binding, four-helix bundle, helical hairpin
• 由来する生物種: NICOTIANA TABACUM (COMMON TOBACCO)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16400.66

構造登録者

Hothorn, M.,Scheffzek, K. (登録日: 2006-03-29, 公開日: 2006-03-30, 最終更新日: 2024-10-16)

主引用文献

Hothorn, M.,Scheffzek, K.
Multiple Crystal Forms of the Cell-Wall Invertase Inhibitor from Tobacco Support High Conformational Rigidity Over a Broad Ph-Range
Acta Crystallogr.,Sect.D, 62:665-, 2006

PubMed Abstract: Plant acid invertases catalyse the breakdown of sucrose. Their activity is tightly regulated through interaction with specific protein inhibitors. The complex between the cell-wall invertase inhibitor Nt-CIF and its target enzyme is stable only at acidic pH, as found in the plant cell wall. Since the pH in this compartment can be modulated between pH 4 and 6 in planta, the rapid dissociation of the inhibitor-enzyme complex at neutral pH may represent a regulatory event. Here, it is analyzed whether the inhibitory component undergoes structural rearrangements upon changes in the pH environment. Six crystal forms grown at pH 4.6-9.5 and diffracting up to 1.63 Angstrom indicate only small structural changes in CIF. This suggests that complex dissociation at neutral pH is mediated either by rearrangements in the enzyme or by a complex pattern of surface charges in the inhibitor-enzyme binding interface.

PubMed: 16699194
DOI: 10.1107/S0907444906011693

実験手法

X-RAY DIFFRACTION (1.84 Å)