2CHP

Crystal structure of the dodecameric ferritin MrgA from B. subtilis 168

2CHP の概要

• エントリーDOI: 10.2210/pdb2chp/pdb
• 分子名称: METALLOREGULATION DNA-BINDING STRESS PROTEIN, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: dna-binding protein, dps, dodecameric, ferritin, dna-binding, stress protein, dna binding protein
• 由来する生物種: BACILLUS SUBTILIS SUBSP. SUBTILIS STR. 168
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 69587.00

構造登録者

Essen, L.-O.,Morbitzer, A. (登録日: 2006-03-16, 公開日: 2007-04-03, 最終更新日: 2024-05-01)

主引用文献

Schonafinger, A.,Morbitzer, A.,Kress, D.,Essen, L.,Noll, F.,Hampp, N.
Morphology of Dry Solid-Supported Protein Monolayers Dependent on the Substrate and Protein Surface Properties.
Langmuir, 22:7185-, 2006

PubMed Abstract: The morphologies of dry MrgA protein monolayers on different solid substrates prepared by a three-step procedure (adsorption from an incubation solution, rinsing to remove excess salt and protein, and drying) were investigated using atomic force microscopy. MrgA is a dodecameric iron-storage protein which can form hexagonal, two-dimensional (2D) crystalline monolayers on hydrophilic surfaces at low supersaturation. The formation of such two-dimensional crystals is heavily dependent on the pH and the salinity of the incubation solution as well as on the surface properties. Correlation of surface coverage with substrate charge, ionic strength, and pH indicates the dominance of electrostatic effects in adsorption, with the balance shifting between intermolecular repulsion and protein-substrate attraction. Close to the isoelectric point (pI) of MrgA, adsorption to the surface and the formation of 2D crystals are favored. By preparation of self-assembled monolayers of thiols with different end groups on template-stripped gold, the surface properties can be varied easily from high to very low protein affinity. The resulting patterns of the crystalline protein structures are novel and could be a starting point for further scientific study, e.g., solid-supported cocrystallization with DNA, and indeed developments with technological applications, such as mesostructured deposition of MrgA-caged nanoparticles.

PubMed: 16893214
DOI: 10.1021/LA0530182

実験手法

X-RAY DIFFRACTION (2 Å)