2CHK
NMR structure of TLLLLT quadruplex
Summary for 2CHK
| Entry DOI | 10.2210/pdb2chk/pdb |
| Related | 2CHJ |
| Descriptor | 5'-D(*T LCG LCG LCG LCGP*TP)-3' (1 entity in total) |
| Functional Keywords | quadruplex, lna, locked nucleic acid, nucleic acid |
| Total number of polymer chains | 4 |
| Total formula weight | 7969.16 |
| Authors | Nielsen, J.T.,Petersen, M. (deposition date: 2006-03-15, release date: 2006-04-19, Last modification date: 2024-05-15) |
| Primary citation | Nielsen, J.T.,Arar, K.,Petersen, M. NMR Solution Structures of Lna (Locked Nucleic Acid) Modified Quadruplexes Nucleic Acids Res., 34:2006-, 2006 Cited by PubMed Abstract: We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology. PubMed: 16614450DOI: 10.1093/NAR/GKL144 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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