2CH1

Structure of Anopheles gambiae 3-hydroxykynurenine transaminase

2CH1 の概要

• エントリーDOI: 10.2210/pdb2ch1/pdb
• 関連するPDBエントリー: 2CH2
• 分子名称: 3-HYDROXYKYNURENINE TRANSAMINASE, PYRIDOXAL-5'-PHOSPHATE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: plp-enzyme, kynurenine pathway, anopheles gambiae, 3-hydroxykynurenine transaminase, transferase
• 由来する生物種: ANOPHELES GAMBIAE
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 178757.32

構造登録者

Rossi, F.,Garavaglia, S.,Giovenzana, G.B.,Arca, B.,Li, J.,Rizzi, M. (登録日: 2006-03-10, 公開日: 2006-03-28, 最終更新日: 2023-12-13)

主引用文献

Rossi, F.,Garavaglia, S.,Giovenzana, G.B.,Arca, B.,Li, J.,Rizzi, M.
Crystal Structure of the Anopheles Gambiae 3-Hydroxykynurenine Transaminase
Proc.Natl.Acad.Sci.USA, 103:5711-, 2006

PubMed Abstract: In Anopheles gambiae, the vector for the most deadly malaria parasite Plasmodium falciparum, xanthurenic acid (XA) plays a key role in parasite gametogenesis and fertility. In mosquitoes, XA is produced by transamination of 3-hydroxykynurenine (3-HK), a reaction that represents the main route to prevent the accumulation of the potentially toxic 3-HK excess. Interfering with XA metabolism in A. gambiae therefore appears an attractive avenue for the development of malaria transmission-blocking drugs and insecticides. We have determined the crystal structure of A. gambiae 3-HK transaminase in its pyridoxal 5'-phosphate form and in complex with a newly synthesized competitive enzyme inhibitor. Structural inspection of the enzyme active site reveals the key molecular determinants for ligand recognition and catalysis. Major contributions toward inhibitor binding are provided by a salt bridge between the inhibitor carboxylate and Arg-356 and by a remarkable hydrogen bond network involving the anthranilic moiety of the inhibitor and backbone atoms of residues Gly-25 and Asn-44. This study may be useful for the structure-based design of specific enzyme inhibitors of potential interest as antimalarial agents.

PubMed: 16585514
DOI: 10.1073/PNAS.0510233103

実験手法

X-RAY DIFFRACTION (2.4 Å)