2CFX

Structure of B.subtilis LrpC

2CFX の概要

• エントリーDOI: 10.2210/pdb2cfx/pdb
• 分子名称: HTH-TYPE TRANSCRIPTIONAL REGULATOR LRPC (2 entities in total)
• 機能のキーワード: transcription, lrpc, transcriptional regulation, dna binding, ffrp
• 由来する生物種: BACILLUS SUBTILIS
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 131776.16

構造登録者

Thaw, P.,Rafferty, J.B. (登録日: 2006-02-24, 公開日: 2006-03-28, 最終更新日: 2024-05-01)

主引用文献

Thaw, P.,Sedelnikova, S.E.,Muranova, T.,Wiese, S.,Ayora, S.,Alonso, J.C.,Brinkman, A.B.,Akerboom, J.,Van Der Oost, J.,Rafferty, J.B.
Structural Insight Into Gene Transcriptional Regulation and Effector Binding by the Lrp/Asnc Family.
Nucleic Acids Res., 34:1439-, 2006

PubMed Abstract: The Lrp/AsnC family of transcriptional regulatory proteins is found in both archaea and bacteria. Members of the family influence cellular metabolism in both a global (Lrp) and specific (AsnC) manner, often in response to exogenous amino acid effectors. In the present study we have determined both the first bacterial and the highest resolution structures for members of the family. Escherichia coli AsnC is a specific gene regulator whose activity is triggered by asparagine binding. Bacillus subtilis LrpC is a global regulator involved in chromosome condensation. Our AsnC-asparagine structure is the first for a regulator-effector complex and is revealed as an octameric disc. Key ligand recognition residues are identified together with a route for ligand access. The LrpC structure reveals a stable octamer supportive of a topological role in dynamic DNA packaging. The structures yield significant clues to the functionality of Lrp/AsnC-type regulators with respect to ligand binding and oligomerization states as well as to their role in specific and global DNA regulation.

PubMed: 16528101
DOI: 10.1093/NAR/GKL009

実験手法

X-RAY DIFFRACTION (2.4 Å)