2CFQ

Sugar Free Lactose Permease at neutral pH

2CFQ の概要

• エントリーDOI: 10.2210/pdb2cfq/pdb
• 関連するPDBエントリー: 1M2U 1PV6 1PV7 2CFP
• 分子名称: LACTOSE PERMEASE, MERCURY (II) ION (2 entities in total)
• 機能のキーワード: transport, lactose permease, transport mechanism, lactose/h+ symport, sugar transport, transmembrane, formylation
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P02920
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47487.75

構造登録者

Mirza, O.,Guan, L.,Verner, G.,Iwata, S.,Kaback, H.R. (登録日: 2006-02-22, 公開日: 2006-03-13, 最終更新日: 2023-12-13)

主引用文献

Mirza, O.,Guan, L.,Verner, G.,Iwata, S.,Kaback, H.R.
Structural Evidence for Induced Fit and a Mechanism for Sugar/H(+) Symport in Lacy.
Embo J., 25:2038-, 2006

PubMed Abstract: Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

PubMed: 16525509
DOI: 10.1038/SJ.EMBOJ.7601028

実験手法

X-RAY DIFFRACTION (2.95 Å)